s100ß a neurotrophic factor mainly released by astrocytes, has been implicated in the pathogenesis of bipolar disorder. thus, lithium may exert its neuroprotective effects to some extent through s100ß. furthermore, the possible effects of lithium on astrocytes as well as on interactions between neurons and astrocytes as a part of its mechanisms of actions are unknown. this study was undertaken...

background: in vitro model studies are becoming increasingly popular for experimental research designs. they include isolation and expansion of cells of a particular tissue, such as the nervous tissue which contributes to understanding the underlying mechanisms in many pathologies. it enables  the scrutinization of intracellular signaling pathways responsible for cell death. objectives: in the ...

introduction: antibiotic supplements are regularly used in neuronal culture media to control contamination however, they can interfere with the neuronal excitability and affect electrophysiological properties. therefore, in this study, the effect of penicillin/streptomycin supplements on the spontaneous electrophysiological activity of hippocampal pyramidal neurons was examined. methods: electr...

abstract background and aims: to identify and isolation of viruses a susceptible cell is need which can be primary or line. the use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. irkhbk cell line was originated from primary cells of bovine kidney by continuous passage. the aim of this study was to introduce the irkhbk cell lin...

the aim of the present study was to develop an in vitro system for functional investigation of ovarian follicular cells in sterlet, acipenser ruthenus. oocytes for the primary culture were obtained from the ovaries of a 6 years old sterlet 729 g in weight and 47 cm in total length. the oocytes were in advanced vitellogenesis stage (pi >10). a part of the ovary (containing about 300 follicles) w...

Mouse hepatocytes in primary culture were characterized. Hepatocytes were isolated by the two-step hepatic portal vein perfusion method described previously. An optimal cell attachment of 43% was noted after 2 h incubation in 10% fetal bovine serum. Minimal attachment (less than 7%) occurred in serumless medium. Serum concentrations above 10% and attachment durations greater that 2 h resulted i...

results local level of primary health care system in iran had mechanical structure, but in contextual dimensions the results showed different types. there was a significant relationship between structural and contextual dimensions (r = 0.642, p value < 0.001). goals and culture dimensions had strongest effects on structural dimensions. background in recent years, family physician plan was estab...

background: current anti-cancer drug therapy results in systemic side effects due to non-specific uptake by normal healthy noncancerous tissues. to alleviate this difficulty, many attempts have been devoted to the development of new delivery systems such as polymeric nanoparticles (nps). in this study, we prepared icd-85 nps based on sodium alginate and analyzed the cytotoxic activity of icd-85...